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Image Search Results
Journal:
Article Title: Melanoma inhibitor of apoptosis protein (ML-IAP) is a target for immune-mediated tumor destruction
doi: 10.1073/pnas.0530311100
Figure Lengend Snippet: Vaccination-augmented anti-ML-IAP antibody titers and induced isotype switching. (A) Pan-IgG. (B) IgG4. The ELISA was performed with recombinant GST-ML-IAP protein and K030 sera diluted 1:10,000 for the pan-IgG determinations and 1:100 for the IgG4 measurements. Small arrows denote vaccinations. The peak pan-IgG and IgG4 levels were 4-fold and 10-fold higher than the respective values at study entry. K030 sera also recognized GST-ML-IAP in Western analysis. Reactivity to Candida antigens was not consistently affected by therapy (data not shown).
Article Snippet: A polyclonal goat anti-human pan IgG (Jackson ImmunoResearch, 1:1,000 dilution), monoclonal mouse anti-IgG1 (Zymed, 1:500 dilution), or
Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Western Blot